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Addgene
Showing: 1 - 20 of 26591 results

  1. pJK545

    Plasmid
    #74225
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    Use
    Tags
    Expression
    Bacterial
    Mutation
    contains a silent mutation in Ala63 codon
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    74225-plasmid-map-sequence-id-142021

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  9. pDA125

    Plasmid
    #74249
    Purpose
    Intermediate cloning vector, with MCS2 containing inducible unstable peptide driven by pSTL1 (UbiY 2xNLSs SZ1).
    Depositor
    Serge Pelet
    Article
    Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10
    Insert
    Inducible peptide of the dPSTR
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    pSTL1
    Availability
    Academic Institutions and Nonprofits only
    74249-plasmid-map-sequence-id-140105

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  10. pDA186

    Plasmid
    #74250
    Purpose
    Intermediate cloning vector, with MCS2 containing inducible unstable peptide driven by pGPD1 (UbiY 2xNLSs SZ3).
    Depositor
    Serge Pelet
    Article
    Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10
    Insert
    Inducible peptide of the dPSTR
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    pGPD1
    Availability
    Academic Institutions and Nonprofits only
    74250-plasmid-map-sequence-id-139759

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  11. pDA171

    Plasmid
    #74251
    Purpose
    Intermediate cloning vector, with MCS2 containing inducible stable peptide driven by pSTL1 (Venus 2xNLSs SZ1).
    Depositor
    Serge Pelet
    Article
    Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10
    Insert
    Inducible peptide of the dPSTR
    Use
    Tags
    Venus
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    pSTL1
    Availability
    Academic Institutions and Nonprofits only
    74251-plasmid-map-sequence-id-139758

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  13. pJK556

    Plasmid
    #74277
    Purpose
    Produces formylglycinamide ribonucleotide amidotransferase, N-terminal His6 tag (H6EcPurL)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes alanine-1246 to serine, numerous silent m…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    74277-plasmid-map-sequence-id-139780

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  17. pJK122

    Plasmid
    #74295
    Purpose
    Produces Acetobacter aceti 1023 hypothetical acetic acid resistance protein B, cleavable N-terminal NusA fusion
    Depositor
    T. J. Kappock
    Insert
    hypothetical acetic acid resistance protein B
    Use
    Tags
    NusA
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    74295-plasmid-map-sequence-id-141881

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  19. pSG4K5

    Plasmid
    #74492
    Purpose
    For cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2
    Insert
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Availability
    Academic Institutions and Nonprofits only
    74492-plasmid-map-sequence-id-140493

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  20. pCas9(D10A)

    Plasmid
    #74495
    Purpose
    Nicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10A
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2
    Insert
    Cas9 D10A
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    D10A
    Promoter
    pTeto
    Availability
    Academic Institutions and Nonprofits only
    74495-plasmid-map-sequence-id-140495

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Showing: 1 - 20 of 26591 results